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As a reporter gene for evaluating and optimizing the protocol for transient expression of the introduced gene in planta, we monitored transient expression of β-glucuronidase (GUS) in alfalfa leaves.
We observed four distinct cerebral NADH species under physiological conditions with high spatial resolution, and we monitored transient alterations in the relative amounts of these species under anoxic conditions with relatively high temporal resolution.
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The challenge of developing adequate experimental methods for monitoring transient structure is discussed.
A main focus of this work is set on the ability of OCT to monitor transient processes with temporal resolution in a second to minute scale.
The experimental setup consisted of monitoring transient groundwater fluxes generated by a multiple step pumping test, which was undertaken in the alluvial aquifer of the River Meuse, Liège (Belgium).
Another use for this type of spectral editing can be in monitoring transient biomolecular interactions.
By monitoring transient kinetics under multiple-turnover conditions, one can often obtain kinetic information not available during steady-state measurements.
Treatment of HEK 293T cells with RCS1 enabled monitoring transient increases in cytosolic Cu+ that originated from intracellular stores after stimulation with ascorbate.
Zheng and colleagues developed a new ultrasound system for monitoring transient depth-dependent osmotic swelling and solute diffusion in articular cartilage [ 14- 16].
Future investigations will explore the viability of this technique by monitoring transient features of each component during exposure to various metabolic inhibitors.
We have investigated the possibility that variations in the level of intracellular Ca2+ in excitable cells might be induced as an artifact of the incoherent illumination that is being used to monitor transient responses.
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