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Doxorubicin accumulation in tumour cells can be monitored through fluorescence imaging if the tumour is located on the skin surface.
Folding was monitored through fluorescence, susceptibility to base-catalyzed cleavage, nuclear magnetic resonance, and indirectly through SAM binding.
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Unlike traditional methods of measuring cell proliferation, the CFSE cell division assay was employed to detect the cell division arrest induced by DN in A549 cells.As CFSE fluorescence intensity is reduced by one half at each cell division, the proliferation of CFSE-labeled cells either untreated or treated with DN can be monitored through such fluorescence intensity.
The activity of FBAs was monitored through a fluorescence-based assay measuring the increase in fluorescence due to conversion of resazurin to resorufin via diaphorase when coupled with the oxidation of NADH to NAD+.
The resultant free HR3 then enters the nucleus and activates reporter gene transcription, which is monitored through the appearance of fluorescence in the transfected cells.
The Aedans-fluorophor was excited at 350 nm and change in fluorescence was monitored through a 408 nm cutoff filter.
The mant group was excited at 360 nm and fluorescence was monitored through a 405 nm cut-off filter.
Upon binding (λex 290), the change in fluorescence was monitored through a 400 nm long-pass filter using the Kintek Auto-SF stopped flow (Kintek), and the resulting traces were fit to a double exponential equation (Equation 5, above).
3D cell migration and cell transfection were monitored through time-lapse video microscopy and fluorescence microscopy.
The EET process was monitored through steady-state and time-resolved fluorescence spectroscopic study.
The DNA fluorescence of PI-stained cells was analyzed by excitation at 488 nm and monitored through a 630/22 nm band-pass filter using a FACScan flow cytometer (Becton-Dickinson, Frankton Lakes, NJ).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com