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Fmoc deprotection was performed in 20% piperidine in DMF and monitored through absorbance at 364 nm.
Their presence was monitored through absorbance at 280 nm.
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The aggregation of lipid vesicles was monitored through visible absorbance measurements.
The integrity of the protein can be monitored through the bacteriochlorin absorbance bands at 803 and 760 nm, which show distinctive decreases in amplitude as the protein unfolds (see inset to Figure 5 A; note that the band at 865 nm photobleaches immediately on strong illumination).
The mixture was incubated at 25 °C for 30 min and activity monitored through the production of 1,2-benzoquinone by absorbance readings taken at 470 nm.
Second, a short flash of light was used to form the charge-separated state P865+Quinone−, and the rate of recombination of this radical pair was monitored through the recovery of bleaching of the same ground-state absorbance band of P865.
Catalase activity in cell extracts was monitored through the decomposition of hydrogen peroxide by following the decrease in absorbance at 240 nm [18].
The formation of AgNPs was monitored through visual observation of the change of colour, and measurement of the absorbance spectrum of the reaction mixture using UV Visible spectrophotometer (Genesys 10 UV Thermoelectron Corporation, UK).
The growth of the sprayed films was monitored through microbalance (QCM) and ultraviolet visible (UV Vis) absorption spectroscopy, which reveal a higher mass and absorbance per deposited layer of K[Eu dbm 4] film.
Larvae were monitored through to pupation.
Protein elution was monitored by absorbance at 280 nm and confirmed by SDS polyacrylamide gel electrophoresis.
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