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We used in vitro synthesized dicistronic viral transcripts that contained two consecutive reporters Rluc and Fluc, and monitored their expressions in RRL and compared the results obtained with those from the Giardia trophozoites transfected with the same transcripts [44] (Fig. 2).
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To rule out the possibility that some of these constructs were not expressed in the germline, we monitored their expression pattern and level by GFP fluorescence (Fig. 6A).
We thus monitored their expression during ES epidermal differentiation as compared to undifferentiated ES cells.
We monitored their expression levels in human melanoma cell lines compared to normal human epidermal melanocytes (NHEM).
We monitored their expression in colon cancer cell lines including SW480, SW620, HCT116, HT29, RKO, and detected relatively high expression in HCT116 and SW620 cells (Supporting Information Fig S3).
Based on bioinformatics analysis of the apple genome, we identified three apple GAD genes and then monitored their expression in various plant organs, including apple fruit.
We selected the remaining five genes and monitored their expression in untreated and fungal-inoculated Col-0, Te-0 and Kas-1 leaves.
Here, we randomly selected four Helitrons and monitored their expression via RT PCR analysis of RNA extracted from etiolated roots and shoots.
To verify the RNA-seq data by WISH, we selected a representative gene from each of the major expression categories and monitored their expression in chd4, p53 and zfp-1 RNAi backgrounds.
Since in the OE the functional activation of ORs requires Gα olf and Adenyl Cyclase III (Adcy3), we monitored their expression in our datasets showing that both signaling molecules were present in A9 and A10 mDA neurons (Additional file 2: Figure S1a).
In order to assess whether UBE2L6 and TRIM25 are induced as rapidly as ISG15 at the protein level, we monitored their expression by immunoblot at 3 hr post infection.
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