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To address this question we first monitored the increase of body weight in our strain of Nscl-2 mutant mice.
incorporated both diamond and an acceptor into a polymer matrix, and they also bleached some of the dye molecules and monitored the increase in fluorescence.
To determine the salt sensitivity of kon, we monitored the increase in 2-AP fluorescence under irreversible second-order conditions, in which both the enzyme and DNA were mixed in equal molar amounts (400 or 600 nM) using increasing concentrations of KGlu (36 150 mM).
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kcat and Km values of the wild-type and variant enzymes were determined by measuring the reaction rate at 25 °C, by monitoring the increase in A340 (ε = 6,220 M−1 cm−1).
Each library was screened (approximately 200 colonies) using the criterion of rate of formation of NADH from NAD+, by monitoring the increase in A340.
Reactions were stopped with cold PBS and live, single, CD11c+MHCII+ cells were analyzed by flow cytometry by monitoring the increase in 450 nm fluorescence resulting from CCF4 cleavage and loss of 535 nm emission fluorescence.
Sensitive quantitative detection of DIF was carried out by monitoring the increase of charge transfer resistance (Rct) by increasing the DIF concentration.
This can be achieved by monitoring the increase of the internal resistance of the battery cells over the whole lifetime of the battery.
The enzyme activity was measured by monitoring the increase in absorbance at 470 nm during polymerization of guaiacol into tetraguaiacol.
Images were taken before and after ablation, as well as in 5-second intervals during ablation to monitor the increase in stiffness contrast and extent with time.
The Udh activity was determined photometrically by monitoring the increase of NADH at 340 nm with a Multiskan spectrum spectrophotometer (Thermo Fisher Scientific).
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