Exact(7)
They grew neurons in the scaffold, then successfully monitored the cells' firing activity in response to excitatory neurotransmitters; they observed heart cells on one side of the tissue beating in subtly different ways than cells on the other side; and they monitored pH changes on the inside and outside of a simplified blood vessel, made of rolled construct and smooth muscle cells.
In our ex vivo study we monitored the cells in proliferating culture.
We then monitored the cells for 2 days using time-lapse fluorescent microscopy.
When we monitored the cells after TMV-RNA transfection for 24 h, TMV-positive RNA (red) was found throughout the cells as small, irregularly-sized granules that then accumulated in the nucleus as high-density granules after 48 h.
To examine cell spreading, we expressed our GFP-vinculin variants in Vin −/– MEFs, examined their expression levels by Western blot (data not shown), and monitored the cells using the real-time cell analyzer (RTCA) xCELLigence system.
We challenged the cell lines with either MNNG or H2O2 at the highest doses tested above, and monitored the cells for apoptosis after 0, 1, 2, 3 and 24 h after treatment.
Similar(52)
During the incubation, we monitored the cell growth rate in serum-free medium and compared it to the growth rate of cells maintained under the same conditions in serum-containing medium.
Following release from a G1/S block, we harvested cells and assayed luciferase activity, and we monitored the cell cycle profile by flow cytometry.
To illustrate how the reprogramming proceeds, we monitored the cell state transition for the wild-type and reprogrammed cells.
Because we monitored the cell viability before electrophoresis, we can exclude that cell damage accounts for the low values.
Subsequently, we monitored the cell viability of L1210 and L1210FR cells after treatment with targeted 47·oxaliplatin versus untargeted CB[7]·oxaliplatin.
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