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To follow the functional impact of UDCA on ADAM17-mediated signaling, we monitored the activation status of mitogen-activated protein kinase 1 and 2 (ERK1/2), the downstream signal of TGFα binding to the EGF receptor [ 31].
To determine whether this reduced level of γ-H2A.X was due to inefficient activation or faster de-phosphorylation (which normally occurs later in the DDR pathway)25, we monitored the activation of γ-H2A.X after DSB induction by phleomycin.
We monitored the activation of two populations of CaM in the spine.
In order to determine whether PLCγ1 is a downstream target of FGF-2 in HUVEC we monitored the activation of the enzyme by metabolic labelling of cells and analysis of the total amount of inositol phosphates generated upon FGF-2 stimulation.
We then monitored the activation status of Chk1 in isolated nuclei treated with aphidicolin.
To evaluate the basis of Te-0 resistance to G. cichoracearum, we monitored the activation of defence markers in this interaction.
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These activity reporters have also been utilized to monitor the activation mechanism of PKD in cardiomyocytes, which involves its release from association with AKAP-lbc and translocation into nucleus [ 10].
Flow cytometry was used to monitor the activation of platelets and detect the binding of complement proteins to non-activated platelets or platelets activated with 33.5 µM TRAP.
In their in vitro evaluation by monitoring the activation of caspase-3 in Schwann cells, peptide derivatives demonstrated retention of peptide activity reducing TTR aggregates (TTRagg) cytotoxicity upon conjugation and a greater plasma stability than the parent free peptide.
Cellular signal transduction occurs in complex and redundant interaction networks, which are best understood by simultaneously monitoring the activation dynamics of multiple components.
In Steps 5 and 6 you will use these skills to actually monitor the activation of habitual self-schemas as they occur in your daily life.
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controlling the activation
trace the activation
controlled the activation
monitoring the activation
monitored the fieldwork
monitored the food
monitored the duration
monitored the pressure
monitored the appearance
monitored the effect
monitored the issue
monitored the gathering
monitored the word
monitored the flotilla
monitored the intervention
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