Your English writing platform
Discover LudwigSuggestions(1)
Exact(1)
Therefore, we monitored sodB expression by qRT-PCR.
Similar(59)
Examination of sodB expression in 26695 revealed a smaller protected fragment than originally expected.
While sodB expression remained unchanged in G27 under iron depletion shock conditions, a two-fold decrease in sodB expression was observed in the "−5 bp swap" strain (Fig. 5A).
Again, there was no decrease in sodB expression in G27, indicating that growth phase is not responsible for the differences in our results.
This change is Fur-dependent as there is no change in sodB expression under either iron depletion condition in the absence of fur.
Because the decrease in sodB expression in 26695 is most pronounced in exponential phase, only results of RPAs performed using exponential phase RNA are shown.
However, as shown in Fig. 1A, the addition of iron chelator resulted in no change in the level of sodB expression.
apo-Fur dependent changes in sodB expression are not observed under iron deplete conditions in H. pylori strains G27, HPAG1, or J99.
As shown in Fig. 4, neither J99 nor HPAG1 displays the expected decrease in sodB expression [1]; both behave similarly to G27 (Fig. 4A).
While the fold decrease in sodB expression in the "−5 bp swap" strain under iron-limited growth conditions did not reach 2-fold, it was consistently higher than its G27 counterpart (data not shown).
For WT 26695, a 2-fold decrease in sodB expression was achieved under both iron-limited growth and iron-depletion shock conditions, which agrees with the previous report [1].
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com