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We then treated OT-II and OT-II.c-FLIPL Tg mice with OVAp323-339 peptide or with PBS, and monitored positive and negative selection of thymocytes in these mice.
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We chose to monitor positive radical variations (+6, +7 and +8. magnitudes) as they are expected to result in significant structural and functional changes on the protein, thereby correlating with molecular adaptation and positive selection [ 71, 72].
These eased controls require that EU member states, as a minimum, intensively monitor positive flocks through testing of fallen stock and animals leaving the flock for slaughter, but with movement restrictions limited to export interdiction [ 18].
The evaluation of immature red blood cell (RBC) parameters, including the number and hemoglobin content of reticulocytes, can be useful for monitoring positive adaptations to training or for identifying the use of prohibited substances to stimulate bone marrow production.
The samples were monitored in positive mode, resulting in species of 1 mass unit higher than the neutral molecules.
The acidic extracts were monitored for positive ions and the basic extracts were monitored for negative ions in independent injections using separate acid/base dedicated 2.1×100 mm Waters BEH C18 1.7 µm particle columns heated to 40°C.
Gβ was monitored as positive Gαi3-binding control.
In assumed negative farms that were not continually monitored, more positive samples were found with ELISA II to IV.
Phosphorylation of CREB or ERK was simultaneously monitored as positive controls of Gs- and Gi-signaling, respectively.
All transitions were monitored in positive ion mode with a dwell time of 10 ms and with Q1 and Q3 set to unit resolution.
The LC runs were monitored in positive ion mode by sequentially recording survey MS scans (m/ z 400 2000), in the ICR cell, while three MS2 were obtained in the ion trap via CID for the most intense ions.
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