Sentence examples for monitored localization of from inspiring English sources

The mfGFP successfully monitored localization of the fusion protein in living cells, isolated the protein complex in native form with a high purity, and detected the protein in immunofluorescence and immuno-electron microscopy (EM).

To test this, we monitored localization of C-terminally GFP tagged eS26 (eS26-GFP) in WT and yrb2Δ cells.

To this end, we monitored localization of 40S subunits in Tsr2-depleted cells using the established reporter uS5-GFP (yeast Rps2-GFP; Milkereit et al., 2001).

We monitored localization of the MDH proteins in different subcellular compartments by expressing recombinant genes that encoded the peroxisomal or cytosolic mutant or the wild-type protein fused to the eYFP fluorescent protein tag at their N-termini.

Furthermore, to monitor localization of the nanoemulsions after application of the cell culture, the cell images were monitored via fluorescence microscope after FITC labeling.

To monitor localization of the endogenous protein in tissues, we generated an affinity-purified polyclonal antibody against FKH.

All the fusion proteins demonstrated appropriate cellular localization, indicating that mfGFP can monitor localization of the fusion partner as is the case with wild type GFP.

The fluorescent GFP signal allowed us to monitor localization of these constructs following cyst maturation.

We used 18S and 28S rRNA FISH and S6-dendra2 overexpression to monitor the localization of rRNA; sensorin, β-thymosin and EF1α FISH to monitor localization of three mRNAs, including one transcriptionally induced mRNA; and Staufen-dendra2 overexpression to monitor localization of an RBP previously reported to be involved in mRNA localization in neurons (Lebeau et al., 2011).

To be able to monitor localization of the proteins, an enhanced green fluorescence protein (eGFP) tag was fused to the amino terminal end of both HMGA2 proteins.

Since QD can be detected by multi-photon fluorescence microscopy [19], this technology could be applied to monitor localization and aggregation of Aβ in brain.