Sentence examples for monitored localisation from inspiring English sources

In order to test whether SVSPs, which are potentially secreted into the host cell cytoplasm, have a tropism for specific host cell structures or compartments, we expressed TP03_0882 lacking the predicted N-terminal signal peptide in various mammalian cell lines and monitored localisation by immunofluorescence microscopy.

We monitored p53 localisation by immunofluorescence staining with four different anti-p53 antibodies.

As a first step, we transfected 293T cells with the pEGFP-N1 vector or with pEWS-Oct-4B-EGFP and carefully monitored the localisation of green fluorescence.

First, we monitored the localisation of the microtubule-associated protein 1A/1B-light chain 3 (LC3), a protein necessary for autophagosome formation, and Snail.

Next, we monitored the localisation of two proteins involved in transcriptional silencing, namely Sir3p and Rap1p, during chronological aging, because the redistribution of the Sir complex from the telomeres to the nucleolus has been previously observed during replicative aging (Sinclair et al. 1997).

The intrinsically fluorescent green fluorescent protein has been used in many laboratories as a cytological marker to monitor protein localisation in live cells.

The cells were immunostained to monitor the localisation of ERGIC membranes and procollagen VII.

Before the SC and IC injection, we transduced these breast CSCs-like through a luciferase-lentiviral vector (Luc-CSCs-like) in order to monitor their localisation in vivo.

The CSCs-like localisation was monitored by in vivo luciferase imaging.

Of these, only multiphoton calcium imaging currently allows precise three-dimensional localisation of each individual monitored neuron within the region of tissue studied, in the intact brain.

Activity was monitored by assessment of the proteins' subcellular localisation and by their ability to activate a co-transfected reporter gene for their transcription factor target SRF.