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In order to define the efficacy of these pharmaceutical tools in MADA cells, we considered the labelling pattern of tri-H3K9 as a parameter, and monitored its staining before and after drug treatment.
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Transgenic mice carrying these constructs were made and expression of the construct was monitored by staining for β-galactosidase activity.
The adherence was monitored by staining with crystal violet (CV) [47].
Cytopathic effect was monitored by staining viable cells with crystal violet [58].
Osteoclast differentiation of BMM was monitored by staining for the presence of TRAP and changes in cell morphology (Fig. 3C).
We also monitored dpERK staining in stage 10 embryos, when the EGFR signalling pathway becomes required for the invagination of tracheal cells and the formation of tracheal placodes.
Mitotic cells were monitored by staining with MPM-2, a mitosis-specific marker.
GUS expression was monitored by staining according to Jefferson et al. [ 20].
CD34 expression was monitored by staining with anti-human CD34-FITC (clone 581, BD Pharmingen #555388, Heidelberg, Germany).
Degree of digestion was monitored by staining SDS-PAGE gels with silver nitrite and Coomassie Brilliant Blue.
Cell viability was monitored by staining cells with propidium iodide (0.05 mg/ml) acquiring fluorescence through a 670 LP filter.
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