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To test directly whether mAL input onto P1 causes hyperpolarization, we expressed ArcLight (Cao et al., 2013), a fluorescent voltage sensor, in P1 neurons and monitored its fluorescence using 2-photon imaging.
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We therefore transfected neurons with GFP-tagged LIMK1 and monitored its diffusibility by Fluorescence Recovery After Photobleaching (FRAP) using time-lapse imaging.
For an assessment of the arising temperature variations, the thermosensitive optical property of RhB was utilized by monitoring its temperature dependent fluorescence intensities.
Further, we illustrated the use of E2 (PI3Kα/IC50 = 0.068 μM, T47D cell viability: IC50 = 0.9 μM) to block cancer cell proliferation and to monitor its subcellular localization by fluorescence microscopy.
This fact makes it possible to evaluate the tissue photodegradation level via monitoring of its fluorescence spectra as a function of the irradiation time and suggests that the Harderian glands provide an excellent experimental model for the study of the photodynamic process in vivo.
This feature allows the Dox release to be tracked by monitoring the recovery of its fluorescence in real time.
Also, the ability of the device to detect metal ions in DMF and in water/DMF (3:1, v/v) at different pHs has been evaluated by monitoring the changes of its fluorescence intensity.
The selectivity of the pH sensor toward protons in commonly used buffer solutions and in the presence of transition metal cations (Co2+, Cu2+, Fe3+, Ni2+, Pb2+, Zn2+, Hg2+ and Ag+) was studied by monitoring the changes of its fluorescence intensity.
The mixture was then incubated at 25 °C, and its fluorescence was monitored using the multimode microplate reader.
Cell labeling was monitored by fluorescence microscopy using a Nikon Eclipse E800 fluorescent microscope.
Kinetics was monitored through fluorescence effects of a fluorophore-quencher pair (carboxyfluorescein (FAM) and black hole quencher (BHQ)).
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