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We infected 25-day-old detached N. benthamiana leaves with zoospore suspensions of isolates representing four different species and monitored infection using ultraviolet (UV) illumination.
After 24 hours, we monitored infection by influenza A viruses using the IFA assay.
We first compared the pathogenicity of five P. brassicae isolates on Brussels sprouts (Brassica oleracea var. gemmifera) and monitored infection and colonization using bright field and fluorescence light microscopy.
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In addition, the diary cards monitored infections, categorized into common cold, pneumonia, pharyngitis, otitis, fever, gastrointestinal infection, and absence from day care institution because of illness.
The new test reveals carriers, helping farmers and veterinarians monitor infection spread over time and track the impact of control measures in ways that were previously impossible.
To monitor infection, an in vivo imaging technique exploiting genetically engineered, luminescent Candida albicans was adopted, and compared with measurements of colony forming units.
We applied [18F]-FDG-PET/CT imaging to monitor infection development in rhesus macaques after MERS-CoV inhalation.
GFP expressed by the adenoviruses was used to monitor infection by adenoviruses (Figure 3B).
We used a similar assay to monitor infection of insect cells, though infection times were longer due to slower virus replication, possibly caused by the lower temperature.
Because induction of iPSCs requires use of lentivirus with high transduction efficiencies, a drug-inducible GFP-expressing lentivirus was added in all transduction experiments to monitor infection efficiency.
Our results also highlight the importance of monitoring infection density dynamics in both males and females to assess the efficiency of Wolbachia-based control strategies.
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