Analytes and IS were separated by a HILIC column with the mobile phase composed of 20 mM ammonium formate in water acetonitrile (30 70, v/v, adjusted to pH 3.0 with formic acid) and monitored in multiple reaction monitoring (MRM) mode using a positive electrospray source.
The Xevo TQ was used in the positive-ion electrospray mode and all analytes were monitored in Multiple Reaction Monitoring (MRM) using nominal mass resolution.
The gradient was 10% A to 70% A in 5 min, followed by 70% A in 10 min, continued to 90% A in 30 min, and 90% A was held for another 5 min. Adducts were monitored in multiple reaction monitoring (MRM) mode.
These mass filters prevent monitoring of multiple fragments in multiple-reaction monitoring (MRM -type experiMRM -typed thus prexperimentstificandon, or cause thusfew data precludeo be obtaidentificationpeak, and thus preclude quantitatior.
Quantification of fentanyl in plasma was done using online solid-phase extraction liquid chromatography coupled with mass spectrometry (Micromass Quattro Premier; Waters, Milford, MA, USA) in multiple-reaction monitoring mode [11].
An ultra performance liquid chromatography-triple quadrupole-linear ion trap mass spectrometry method in multiple-reaction monitoring mode was developed for the rapid determination of 12 bioactive compounds in the leaf and stem of Andrographis paniculata (A. paniculata).
Acquisition took place in multiple-reaction monitoring mode, with two transitions per compound (one for quantification and one for confirmation) in positive ionization mode.
Ionization of the analytes was obtained by electrospray in negative mode and acquisition was performed in multiple-reaction monitoring mode, following the reaction m/z: 250.1 → 121 and 257.1 → 128.1 for DHMBA and DHBMA-D7.
The protonated molecules, [M + H]+, the precursor ions and the diagnostic product ions were monitored in the multiple reactions monitoring (MRM) mode.
The protonated molecules [M + H]+ was identified as the precursor ions and the diagnostic product ions were monitored in dynamic multiple reactions monitoring (MRM) mode.
