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For breeder farms, hens were kept in separate but very similar houses, and monitored for mortality, morbidity, egg laying and hatchability of chicks.
Three weeks post vaccination mice were challenged i.n. with a lethal dose of live A/PR8[H1N1] (6×102 PFU) and monitored for mortality and clinical signs using 30% body weight loss as the end point (Figure 2).
They were also monitored for mortality and signs of WFS.
Experiments were statically renewed every 24 hr and monitored for mortality and tissue malformations.
The animals were monitored for mortality, clinical symptoms, and any adverse reaction of the test material.
Aquaria were monitored for mortality three times a day over a period of 6 days.
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Findings also suggest managers could improve chances for sustaining resilience by responding to rainfall in multiple seasons, monitoring for mortality events, and establishing contingency plans for various types of drought.
For the broiler chicks, houses were monitored for chicken mortality, weight gain and feed conversion.
Infected BALB/c mice were monitored for morbidity, mortality and viral replication in organs as trachea, lungs, heart, kidneys, liver, brain and spleen.
Egg patches were monitored for larval mortality on days 4, 8,12, and 19 of development, and photographs taken of larvae developing in control and treatment comb were mapped using Microsoft Paint 2007.
To determine whether chickens immunized with single or multiple DNA vaccines were protected from a lethal challenge of a heterologous HPAI H5N1 virus, vaccinated chickens were inoculated with 20 LD50 of highly pathogenic A/Vietnam/1203/2004 heterologous virus intranasally using standard methods [25], [40] and monitored for morbidity, mortality, viral shedding and serum antibodies.
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