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Putative Helitrons from each of these two screens were monitored for expression.
Selected Helitrons were monitored for expression via in silico EST analysis.
The macrophage response to infection and activation by immune stimulants can be effectively analysed by microarray analysis allowing thousands of genes to be monitored for expression in parallel.
Three treated mice were monitored for expression of luciferase following addition of Dox (200 μg/ml) (days 4, 15, and 71) and removal of Dox (day 32) from drinking water.
Two types of eQTLs are expected: cis-eQTLs that are located at the same locus that the gene monitored for expression (structural gene) and trans-eQTLs that are located at another locus.
For titer detection, BHK-21 cells were infected with serial dilutions of activated virus and monitored for expression of antigen by X-gal staining (VRP-lacZ) or immunofluorescence assay (e.g., VRP-THc) as described previously.
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To test this, we generated HeLa cells expressing FKBP-Asf1a and monitored for either expression or depletion of the fusion protein using two different experimental schemes and one experimental control (Figure 8B, TOP).
The cells were sorted for GFP expression 7 days post-infection, and monitored for GFP expression for one month prior to analysis to verify establishment of a persistent infection.
Fourteen initially non-expressing clones were regularly monitored for the expression of RFP by fluorescence microscopy.
48 h post transfection, cells were monitored for GFP expression and selected with puromycin that stably expressed the GFP (a marker that was used to track shRNA mir expression).
Progeny were monitored for transgene expression by PCR analysis.
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