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After incubation, the medium was monitored for dye decolorization.
After the incubation period, aliquots were withdrawn and monitored for dye decolorization.
After the incubation period, 2 ml aliquots were withdrawn, centrifuged at 7000 rpm for 20 min, and monitored for dye decolorization.
After the incubation period, 2 mL aliquots were withdrawn and monitored for dye decolorization as reported by Khan et al. (2014) using Eq. (1); {text{Decolorization }}left( % right), =, left( {left( {{text{AC}} - {text{AT}}} right)/{text{AC}}} right) times 100, (1 where AC is the absorbance of the control and AT is the average absorbance of the test samples.
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As in the 2011 experiments, all fish were individually marked with elastomer dyes and monitored for 2 3 days before the experiment.
The Psychrobacter strains were monitored for their ability to decolorize three different azo-dyes (fast orange, methanil yellow and acid fast red).
HeLa cells were monitored for intracellular ROS generation using a peroxide-sensitive fluorescent probe, 2′, 7′-dichlorofluorescin diacetate (Carboxy-H2DCFDA); or mitochondrial superoxide sensitive dye, MitoSOX™.
Photodamage to liposomes loaded with the fluorescent dye sulforhodamine B (SRB) was monitored in a dye leakage assay.
The assay plates were incubated at 37°C for 6 days, and growth was monitored using resazurin dye, as mentioned above.
For the active in-situ spectroscopic approach, a home-made set-up has been appropriately designed and developed for real time monitoring the dye adsorption on the ZnO-based photoanodes, whereas the standard passive dye loading measurements were performed after the electrodes had been sensitized for different time periods in the dye solutions of different concentrations.
Membrane damage was monitored using fluorescent dyes and fluorescence microscopy.
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