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NIH 3T3 and 208F cells were transfected using calcium phosphate as previously described [ 15] and were monitored for appearance of transformed foci of cells for up to a month after transfection.
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Additionally it could be used to continuously monitor for appearance of new food contaminants as a complement to the specific targeted analysis that is today's foundation of food safety analysis.
Infections were monitored for the appearance of lesions and diminished growth in secondary leaves for 16 days post-inoculation.
ZDF rats were allowed to acclimate for 1 wk and glucose levels were monitored for the appearance of diabetes (>200 mg/dL).
The lysate was used to infect fresh BHK cell monolayers and the cultures were monitored for the appearance of virus growth.
Therefore, it is essential that domestic animal populations with small effective population sizes are continuously monitored for the appearance of recessive defects, so that selection against the deleterious alleles can be implemented as early as possible.
All groups of swine were challenged with 105.0 TCID50 (50% tissue culture infective dose) of FMDV (O/Taiwan/97) and monitored for the appearance of FMD symptoms for two weeks.
Recent changes to the atomoxetine SPC highlight that anxiety was not worsened in clinical trials but that patients being treated with atomoxetine should be monitored for the appearance or worsening of anxiety.
Since F1 hybrid FVB x B6 Cd151−/− mice were healthy and did not present any sign of kidney disease onset (monitored for the appearance of proteinuria over a 12 months period, our unpublished data), we conducted the experiments on this hybrid background.
A prospective clinical study of patients in the initial phase of TBE who were monitored for the appearance of the second, encephalitic phase of the disease, showed that an abortive form of TBE (i.e., an isolated initial phase not followed by the meningoencephalitic phase), as seen in the third patient in our study, is a rare event (10 ).
DOI: http://dx.doi.org/10.7554/eLife.00013.004 > -wrap-foot> To determine which co-isolated bacterial species stimulate rosette colony development in S. rosetta, the RCA cell line was supplemented with 64 independent bacterial isolates from ATCC50818 and monitored for the appearance of rosette colonies.
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