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Cell growth was monitored every hour by measuring cell turbidity at 600 nm using an OD monitor.
E. coli (AS19) growth was monitored every hour in the absence or presence of resveratrol or PNA.
LD-entrained larval brains were dissected and mounted at ZT1 or ZT13, and PDF (2 μM), DMSO or PDF (2 μM) + TTX (100 nM) was applied at ZT2 or ZT14. 3 × 69-VNP (a) and PER-TDT expression (b) in brain explants were monitored every hour by time-lapse microscopy.
Mouse embryos were first cultured to very late 2-cell stage and then monitored every hour.
During this time, they were monitored every hour for the first 6 h after surgery and every 4 h thereafter.
The renewable energy production is also monitored every hour since the year 2010 and has been collected for analysis.
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I slept in the bedroom next to his, monitoring every hour of his day and night, and I treasured our closeness -- in large part because it came so late in his life.
Mice were monitored every 2 hours for 48 hours and then every 4 hours for an additional 24 hours.
Survival rate was monitored every 24 hours throughout 120 hours.
Cell proliferation was monitored every 24 hours for 96 hours by cell counting with a 1/400 mm2 hemocytometer (Hawksley, Lancing, UK), and a sample of cells was taken at each time point for Western blotting and rt-PCR to check the stability of the siRNA knockdown.
In addition, to monitor the kinetics of bacterial growth for MT8148, OD550 values of the bacterial suspensions were adjusted 1.0 × 107 CFU/ml with or without S-PRG eluate and monitored every 1 hour until the bacterial growth reached the stationary phase.
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