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Fluorescence was monitored each cycle during the annealing step, and data were analyzed with SmartCycler software version 2.0d (Cepheid, Sunnyvale, CA, USA).
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The relative levels of these four molecules were monitored at each cycle of the SELEX procedure to verify and standardize the growth conditions of the cells.
The fluorescent DNA binding dye SYBR Green was monitored after each cycle at 80°C.
Blood counts were assessed weekly during therapy; liver, renal function tests and electrolytes were monitored before each cycle.
During the study, patients were monitored before each cycle for medical history, physical evaluation, clinical benefit, serum chemistry and tumour markers, and evaluated for toxicity.
Firstly, the progression of the PCR reaction may be monitored after each cycle rather than at the end, thereby providing a much better quantification assay; secondly, very little DNA is needed; thirdly it is a non-radioactive assay; and finally it can be performed in approximately two hours.
The leaders control the news media, and every village has a family planning officer who sometimes monitors each woman's menstrual cycle.
Quantification of the initial template copy number is possible by monitoring fluorescence at each cycle at a constant temperature.
The MT-PCR-HRM was performed in a Rotor-Gene 6000 (Qiagen, UK) using the following conditions: 95°C for 10 min, followed by 35 cycles at 95°C for 20 s, 57°C for 30 s and 72°C for 50 s; the detection option 'green' was used to monitor fluorescence during each cycle at 72°C.
Amplification of the target gene was monitored every cycle by SYBR Green.
We then monitored cell cycle progression by flow cytometry.
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