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We monitored cell survival over time for the two different surface treatments used to make PDMS hydrophilic.
To investigate this in more detail, we performed a chronological ageing experiment and monitored cell survival (Fig. 6E) and ROS formation (Fig. 6F) when the cultures were kept for a period of two weeks.
Second, we put primary lymphocytes derived from thymus, spleen or lymph nodes in culture and monitored cell survival by Annexin V/PI staining, in combination with cell surface marker staining to identify T and B cells, over time.
We monitored cell survival by counting the cells every 24 h for 6 days continuously.
We also monitored cell survival of all treatments throughout the experiments by trypan blue dye staining.
We next monitored cell survival in the bloodstream and cell lodging in the lungs after tail vein injection.
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To monitor cell survival after transplantation, we then employ molecular imaging techniques that allow repetitive, noninvasive assessment of transplanted ES cell engraftment, viability, and proliferation in small animal models.
It is therefore important to develop methods to monitor cell survival and location after transplantation.
To monitor cell survival in VEGFA-deficient medium, 20,000 cells (confluent) were plated per well and cultured in EGM-2 without addition of serum or VEGFA.
The colony-forming assay used to monitor cell survival entailed irradiation of both types of cells as single-cell suspensions, after which the cells were allowed to adhere and cultured under identical conditions.
To control for the possibility that the observed reduction was because of less GFP-ataxin-1[82Q] expression as opposed to cell death, we repeated the experiment using DsRedII to monitor transfected cell survival.
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monitored cell adhesion
monitored cell confluence
monitored cell density
monitored cell surface
monitored cell growth
monitored cell migration
monitored fish survival
monitored germination survival
monitored cell expansion
monitored cell number
monitored plant survival
monitored cell cycle
monitored cell proliferation
monitored cell uptake
monitored cell morphology
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