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To ascertain if hypotonic solutions altered Merkel-cell volume, we monitored cell shape in three dimensions with fluorescent microspheres attached to the plasmalemma of Merkel cells while perfusing cells with a 20% hypotonic bath solution.
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To monitor cell shape in response to this treatment, bright-field images were acquired every second for 2 min using a Axiovert S100 microscope (Zeiss, Oberkochen, Germany) at 40× magnification.
We monitored cells for both shape and spreading after incubating on collagen type IV substrates.
EPEC utilizes several QS systems to monitor cell density.
We can monitor cell growth and manage side effects better than ever.
These types of devices can capture, track, and monitor cell phone data, even without judicial oversight.
Cerl-GFP-expressing cell shape was monitored over the course of 2 h of live imaging in freshly collected 'in vivo' embryos (c) and in vitro-cultured embryos.
In this paper we take advantage of the developmental synchrony and simplicity of cotyledon development to monitor the microtubule organization and cell shape changes that occur during pavement cell morphogenesis.
Fig. 11 Monitoring culture parameter effect on cell shape changes reviled by online flow cytometry and optic microscopy.
To examine Ca2+ dynamics during cell death, we monitored both changes in GCaMP3 fluorescence and in cell shape at 20 min intervals in time-lapse experiments that extended up to 9 h.
Fluorescence levels were assessed with excitation/emission at 485 nm/505 nm for GFP and 550 nm/570 nm for CF 555 using a BX53 fluorescent microscope with 100-w mercury lamp unit (Olympus, Tokyo, Japan), photographed ( × 400), and counted by viewing the monitor and evaluating cell shapes or CD45 staining.
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