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We next introduced Zfp277 deletion mutants into Zfp277−/− MEFs and monitored cell proliferation.
To confirm these findings with an alternative approach, we monitored cell proliferation by automated cell counting.
We monitored cell proliferation at various time points following treatment of cells with VD3 using MTS assay and trypan blue exclusion.
We monitored cell proliferation quantitatively and in real time in a humidified atmosphere with 5% CO2 at 37°C using an xCELLigence™ RTCA DP instrument (ACEA Biosciences, San Diego, CA, USA).
DOI: http://dx.doi.org/10.7554/eLife.08088.006 To assess whether the reduced TCR activation threshold for proximal signaling events following Csk inhibition had a functional impact on downstream T cell activation, we monitored cell proliferation of CskAS OTII T cells stimulated with plate-bound anti-CD3ε or OVA pMHC tetramer in the presence of anti-CD28 costimulation.
To confirm that the stronger oncogenic effect of pre-miR-146a/G is due to increased abundance of mature miR-146a, we transfected SKMEL-28 cells with increasing amounts of synthetic miR-146a and monitored cell proliferation.
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The fabricated scaffolds using freeze-dry technique were used as a 3D model to monitor cell proliferation and migration.
Furthermore, the integration of the designed microsystem with the multi-well plate reader allowed to monitor cell proliferation in situ during the cultures.
MTS is a very common method for monitoring cell proliferation, and is useful because it specifically detects viable cells, in contrast to many other labeling protocols that do not discriminate between live and dead cells.
To determine if the difference in cellular iron levels between cell lines is due to differential release into the medium, M17, PrPC, PrPΔ51 89, PrPΔ23 89, and PrP102L cells were cultured in the presence of 3H-thymidine overnight to monitor cell proliferation and radiolabeled with 59FeCl3 for 4 hours as above.
To monitor cell proliferation, non-radioactive cell proliferation assays were carried out.
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monitored cell viability
monitored cell adhesion
monitored cell confluence
monitored cell density
monitored cell surface
monitored cell growth
monitored cell migration
monitored cell number
monitored cell cycle
monitored cell uptake
monitored cell morphology
monitored cell survival
monitored cell death
monitored cell shape
monitored cell line
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