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We monitored cell death development in infected leaves of npr1 single and atg5 npr1 double mutants.
We incubated HeLa cells with IpaB and monitored cell death by membrane permeabilization and Annexin V staining using live cell imaging.
To identify potential cell death modality switches upon interference with either cell death pathway, we simultaneously monitored cell death and caspase activity as a function of time.
To additionally clarify whether HHT had any effect on membrane integrity by itself, we monitored cell death in HHT-only treated cells over time (0, 2, 4, 6, 24 hours, using the optimized concentrations determined above).
9- 11 We therefore monitored cell death using an antibody directed against cleaved-Caspase 3 in wing imaginal discs treated with γ-rays (4Gy), Staurosporine (STS - 4μM) and ultraviolet (UV) light (2.5 kJ/m).
To characterize the neurotoxic effects of TBI, we monitored cell death through the TUNEL assay following 12, 24, 48 and 72 h (Supplementary Figure 1b, Figure 1a) using pericontusional cortex (Supplementary Figure 1a) following TBI.
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At present, the development of an effective visualization tool to monitor cell death remains a significant challenge.
Characterization of cell death currently requires the use of indirect markers, which has largely limited the ability to monitor cell death processes inside the cell.
The system efficiently monitors cell death dynamics, being able to detect signals related to specific death mechanisms, namely necrosis or early/late apoptosis, demonstrating a reproducible correlation between the OECT electrical response and the trends of standard cell death assays.
Time-dependent and dose-dependent dynamics of A549 cells exposed to doxorubicin are evaluated by monitoring cell death upon exposure to a range of doses and times that fully covers the protocols used in cancer treatment.
To monitor cell death, cells were stained with propidium iodide (1.25 μg/ml) and analyzed in a FACScan (Becton Dickinson).
More suggestions(15)
monitored cell viability
monitored cell adhesion
monitored cell confluence
monitored cell density
monitored cell surface
monitored cell growth
monitored cell migration
monitored cell expansion
monitored cell number
monitored cell cycle
monitored cell proliferation
monitored cell uptake
monitored cell morphology
monitored cell survival
monitored cell line
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