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They employed an assay using β3-transfected Drosophila S cells and monitored cell adhesion visually.
The xCELLigence system monitored cell adhesion and proliferation for 24 h to allow for attachment.
To rule out the possibility that the results were being masked by differential adhesion to collagen or differential cell recovery when measuring the [H]-thymidine incorporation, we monitored cell adhesion on plastic, on collagen type I and on collagen type IV.
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Cell viability was also analyzed by xCELLigence™ instrument (Roche), which monitors cell adhesion in real-time.
To examine whether Unc5B-mediated loss of cell adhesion requires Rnd1, as was demonstrated for FLRT3 [4], we injected Unc5BΔD mRNA in X. laevis embryos pre-injected with Rnd1 MO and monitored cell deadhesion at blastula stage (Figure 5E,F).
As filopodia play a role in cell migration and adhesion, to monitor cell spreading and attachment ECIS measurements were utilized.
These assays do not facilitate continuous monitoring of cell adhesion, proliferation, migration and invasion, the monitoring of which is vital in determining the effect of the cells external environment.
Therefore, dynamic monitoring of cell adhesion and FA formation could be achieved by fluorescent microscopy utilizing the automated microscopy system Hermes Wiscan.
This result was confirmed by real-time monitoring of cell adhesion and spreading with xCELLigence system over a 24-h period.
However, cell viability assays carried out by flow cytometry and real-time monitoring of cell adhesion showed a marked cell growth reduction for all ALK-positive NB cell lines, including SH-SY5Y and KELLY.
Electric cell-substrate impedance sensing (ECIS) technology is a sensitive technique to monitor and quantify cell adhesion and barrier function of cell monolayer [21].
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