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Transgenic mice carrying these constructs were made and expression of the construct was monitored by staining for β-galactosidase activity.

Annexin V-positive cells in our study displayed a loss of membrane integrity at later stages (monitored by staining with the fluorescent intercalating dye, propidium iodide [PI], which requires structural permeabilization for detection), demonstrating that PS exposure was primarily detected at the outer membrane; cells that are both annexin V- and PI-positive were considered to be dead (Fig. 5).

The adherence was monitored by staining with crystal violet (CV) [47].

Cytopathic effect was monitored by staining viable cells with crystal violet [58].

Osteoclast differentiation of BMM was monitored by staining for the presence of TRAP and changes in cell morphology (Fig. 3C).

The effectiveness of depletion was monitored by staining peripheral blood with a non-competing anti-CD4 mAb (clone RM4-4) antianti CD3 (Clone 145-2C11) using a FACSCalibur® flow cytometer and CellQuest software.

(e) Uptake of 22L or RML PrPSc in siRNA transfected cells was monitored by immunoflourescence staining 18 h post exposure to prions.

Cell surface expression of CD4-anchored sFRP-3-HA protein was monitored by immunofluorescence staining with anti-HA antibody or live GFP expression.

In the light of this observation we investigated whether the different pathogenic mutations in the TPI gene might cause intracellular accumulation of ROS in exponentially growing Δtpi1 yeast cultures monitored by dihydroethidium staining.

For kinetic IFAs the parasites were synchronized within a window of approximately 6 h, monitored by Giemsa staining.

At day 11 after plating on the different aECM, F-actin arrangement was monitored by fluorescence staining.