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Progression of the reaction was monitored by removing 10 µl aliquots that included the suspended beads after 0.25, 0.5, 0.75, 1, 2, 5, and 10 min and mixing them with 30 µl of stop buffer (SB: 90% (v/v) formamide in 1× TBE).
Throughout the experiment, cell growth was monitored by removing a 1-mL sample from each bottle and measuring the OD600 using an Eppendorf BioPhotometer 6131 (Eppendorf, Hamburg, Germany).
Cell growth was monitored by removing samples from the bioreactors and measuring OD600 using a Beckman Coulter DU720 spectrophotometer (1.3 mm slit width) (Beckman Coulter, Inc., Brea, CA, USA) in a 1-mL cuvette.
The specific activity of Av1 in the reaction vial was monitored by removing liquid samples from the assay mixture at specified time points, for immediate transfer into a pH 7.8 standard assay solution containing excess additional Av2 (CR of ∼60).
Appearance of the phenolate was monitored by removing aliquots from the reaction, quenching in a relatively small volume of 1 M NaOH, and measuring the absorbance at 400 nm (with background subtraction at 500 nm to control for instrument drift over the long time periods required to measure the sulfatase activity).
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Since his 1993 brush with cancer, Mr. McCain had been closely monitored by doctors, who periodically removed suspicious moles or lesions from his skin.
The Obama administration cut a deal with the Assad regime ― sponsored and monitored by Russia ― to remove all chemical weapons from Syria and considered that an end to the threat of Syrian chemical attacks.
Given the continuing widespread consideration of ruthenium II) polypyridyl complexes in the detection of DNA, 2 protein, 3, 46 and small molecules, 1 and the difficulty in the synthetic procedures of these inert complexes, the initial screening using an iron II) dynamic library monitored by ESI-MS will remove the requirement for a considerable amount of protracted and difficult synthesis.
The percentage of bacteria crossing into the chamber below was monitored over 72 h by removing aliquots from both top and bottom chambers and enumerating the bacteria in a Petroff-Hausser counting chamber using dark-field microscopy.
Reaction monitoring was performed by removing an aliquot (10 μL) of the reaction mixture, filtering through a pad of cotton, and diluting with CDCl3, after which an NMR spectrum was recorded.
Guzmán's every move inside the prison was monitored by a bracelet, which he removed before making his getaway.
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