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Cell viability on undecanoic acid-terminated porous Si, oxide-terminated porous Si, and oxide-terminated flat (non-porous) Si are monitored by quantification of albumin production over the course of 8 days.

Current approaches for evaluating acyltransferase activity are mainly based on enzymatic assays monitored by quantification of radioactive compounds via thin-layer chromatography [15] [18].

The amount of apotransferrin localized in the cells was monitored by quantification of FITC fluorescence (Ex: 495 ηm, Em: 521 ηm) using fluorimetry.

Cleavage of the internalized CCF2 dye by the translocated SopEM45-TEM-1 fusion protein was monitored by quantification of the fluorescence signal using a Victor3 microplate reader (PerkinElmer) with excitation at 405 nm and emissions at 460 nm (blue fluorescence) and 535 nm (green fluorescence).

The kinetics of adhesion were also monitored by quantification of crystal violet stained-bacteria adhering to the 96-well plates.

The activity of S6K1 can be monitored by quantification of phosphorylation of its substrate, the ribosomal S6 subunit, by intracellular staining with specific phosphorylated S6 antisera.

Similar(54)

Tumour growth was monitored by caliper quantification of diameters.

To characterize the LTB4 function, 5-LO−/− and littermate control mice were infected with L. infantum and the course of infection was monitored by parasite quantification into the organs by a limiting dilution.

Efficiency of the knockdown was monitored by p53 immunoblotting and quantification by ImageJ as previously described [ 15, 52, 53].

Based on QF-PCR results, a total of 108 plasmas were selected from women carrying normal male fetuses so that DNA extraction efficiency could be monitored by real-time PCR quantification of the SRY gene.

The osteogenesis of MSCs was monitored by mineral staining and quantification with alizarin red S. To examine whether Npx affects osteogenic differentiation through HH signaling, the effect of Npx on the expression of IHH, GLI1, GLI2, GLI3 and PTC1 was analyzed with real-time RT PCR.

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