Protein elution was monitored by absorbance at 280 nm and confirmed by SDS polyacrylamide gel electrophoresis.
The elution was monitored by absorbance at 214 nm, and fractions were collected every 1 min.
Diatom growth was monitored by absorbance measurements at 550 nm (Spectronic Educator, Flinn Scientific, Batavia, IL).
The dissolved inclusion bodies were subjected to gel filtration and the elution of protein monitored by absorbance.
The growth of the LbL film was monitored by absorbance versus concentration plots in ultraviolet visible (UV Vis) absorption spectroscopy.
Dye concentrations in the reactor are monitored by absorbance spectroscopy, and the kinetic rate law is determined directly from the batch reactor performance data.
NADPH decrease was monitored by absorbance decrease at 340 nm using a Safire spectrophotometer (Tecan).
The elution profile was monitored by absorbance at 254 nm using G1315D multiple wavelength UV/VIS detector.
The extent of interaction between dyes and each biosorbent was monitored by absorbance measurements, which was then used in isotherm, thermodynamics, and kinetics analysis.
Each endpoint assay was monitored by absorbance at 450 nm (the absorbance-wavelength for the colored product of WST-1 reaction with superoxide) after 20 min of reaction time at 37 °C.
CBMCBHI elution was monitored by absorbance at 280 nm, but it could not be visualized by SDS-PAGE due to its low MW. Figure 1 Expression and purification of CBM-SUMO expressed in E.coli Rosetta (DE3).
