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ATPS were prepared and monitored as previously described.
ATPS for this step were prepared and monitored as previously described.
Mice were monitored as previously described.
Conidiophore was monitored as previously described [32].
Clinical health was monitored as previously described [27].
To examine the effect of cfl1 tMon on AP extension movements, cells of dorsal embryonic shields were marked and monitored as previously described in StdMO- and cfl1 tMO1-injected embryos (Fig. 8B).
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The retinue response was monitored as described previously [17], [21], [68], [69].
Dissolved oxygen in all cultures was monitored as done previously with the ΔarcA strain and the same result was obtained (data not shown) [13].
Air was continuously drawn from an exit hole at back of the den and oxygen consumption monitored as described previously [84].
For vascular permeability measurements, tetramethylrhodamine labeled BSA (TMR-BSA) was injected intravenously and the fluorescence signal in a single optical section containing a vessel of interest was monitored, as described previously [25].
Arabidopsis thaliana plants at the seven-leaf stage were inoculated with plasmids containing partial tandem repeats of CaLCuV DNA-A and DNA-B by biolistic delivery [52] and the course of infection was monitored as described previously [18], [30].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com