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To test the metal leaching, the reaction mixture was separated after a particular time from the reaction mixture at the reaction temperature, and the filtrate was again subjected for reaction for 30 min more, and the products were monitored as before.
Cells were seeded in soft agar and their growth monitored as before.
To test whether this observation was specific for HeLa cells, a number of different cancer cell lines, including Capan1 cells, HT1080wt, Jurkat T cells, IF6 and HT29 cells, were transduced with the same lentiviral shRNA construct and their growth monitored as before.
The depolarizing step protocol described above was performed, and Venus fluorescence was monitored as before.
Coated bees were then placed back in their original cages, and social interactions were monitored as before.
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To solve the riddles, Greenland is being measured and monitored as never before, by satellites, by aircraft and by dozens of down-swaddled scientists who are braving 30-below-zero temperatures and deadly snow-cloaked crevasses that corrugate the slumping edges of the ice cap.
Moreover, the most effective dose of SDH was administered in rats before challenge with toxic dose of sodium nitrite, and MetHb formation was monitored as mentioned before.
XBP1 splicing was monitored as reported before [ 22].
Blood glucose was also monitored as above immediately before and at 15, 30, 45, and 60 min after insulin injection.
Mice were monitored as previously described.
Cd sensitivity was monitored as described above.
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