We devised a sensitive fluorescent reporter assay for monitoring alternative translation initiation.
Large-scale microarray using exon-exon junction probes has been used to monitor alternative splicing events in multi-exon human genes by comparing the junction probe intensity [28].
It was designed as an ambulatory wireless monitoring alternative for patients throughout their stay in the general hospital ward, with no need to recharge the batteries.
The Affymetrix GeneChip® Exon 1.0 ST Array is designed to monitor alternative splicing using more than 1.4 million Probe Selection Regions (PSR) within 1 million exon clusters, which are constructed from various exon annotations [ 21].
The results of this evaluation demonstrate the benefits of our approach, surpassing the monitoring performance and capabilities of cloud monitoring alternatives such as those present in state-of-the-art systems such as Amazon EC2 and OpenNebula.
A combined analysis of three mathematical models, investigating a wide range of monitoring alternatives, but particularly focused on whether viral load monitoring would be cost-effective, found that committing resources to the expansion of ART coverage is likely to generate greater health gains than more complex and expensive monitoring approaches in most circumstances [ 30].
Chinese bank performance should be continually monitored using alternative methods that can shed light on unknown research aspects of banking activity.
Moreover, the aberrant revival kinetics is not limited to glucose and can be monitored when alternative PTS sugars are utilized.
In patients with risk factors for infection (age, glucocorticoid use), IgG levels should be monitored and alternative treatment considered, where appropriate [ 20].
Surrogate markers of brain physiology and metabolism may be monitored through alternative monitoring modalities (transcranial doppler sonography, brain tissue oximetry, jugular bulb oximetry and cerebral microdialysis) [ 50, 51], but these modes are often restricted to a small area of sampled brain and thus may not reflect perfusion and metabolism of other areas of the brain.
As a monitoring alternative, DNA-based methods would greatly increase sensitivity and speed of detection of resistance alleles, but their development is dependent on the identification of resistance alleles.
