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We infected each such well in each set with one of the 10,100 Mtb mutants and monitored alterations in fluorescence induction by automated microscopy.
We have analysed gene expression using whole-genome microarrays and monitored alterations in physiology during acclimation of PSII to elevated growth temperature in Synechocystis sp. PCC 6803.
Thus we monitored alterations in F-actin organisation under different conditions via phalloidin staining, and used such changes to make conclusions of the sensitivity of our cells to injury.
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This allows convenient plate based analysis of glycolytic flux and can be used to monitor alterations in glucose metabolism and to confirm mitochondrial dysfunction.
High-throughput sequencing has been also used to monitor alterations in the expression of miRNAs in virally-infected rice plants (Du et al. 2011a; Guo et al. 2012; Sun et al. 2015).
Unlike histological evaluation, PET imaging also allows for the possibility of serial imaging, which affords the possibility of monitoring alterations in CAIX expression as an indicator of treatment response.
In order to monitor alterations in Runx2 expression in response to these cytokines as a result of TIEG1, three wild-type and three TIEG1 KO calvarial osteoblast cell lines were exposed to TGFβ1 or BMP2 for 2 hours and Runx2 expression levels were monitored by real-time PCR.
Liver was chosen as model organ to monitor alterations in gene expression.
The GSH1 gene was then reintroduced into the cells, and Affymetrix GeneChips were used to monitor alterations in gene expression.
These data show the feasibility to monitor alterations in mitochondrial oxygen consumption in vivo by PpIX-TSLT in a septic rat model.
Photinus pyralis (firefly) luciferase is widely used as a reporter system to monitor alterations in gene promoter and/or signalling pathway activities in vitro.
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