Sentence examples for monitor transgene expression from inspiring English sources
In addition, there might be non-biosafety-related practical reasons to monitor transgene expression.
To monitor transgene expression, the mRFP1 reporter gene was assessed in frozen tumor sections.
With recent advances in the field of molecular imaging, it is now possible to non-invasively monitor transgene expression in cells in vivo using genetically encoded reporters.
The implementation of a quantitative and noninvasive method capable of monitoring transgene expression in living animals repetitively would be useful toward validating the efficacy of any gene therapy strategy.
After transfection of genes into HEK293S cells, we monitored transgene expression in stably transduced cells by immunostaining with a rhodopsin 1D4 (Rho1D4) monoclonal antibody, which recognizes a 9 amino acid TETSQVAPA tag [15] bioengineered into the C-terminus of the OR131-2 receptor.
Monitoring transgene expression over a period of 11 days exhibits decreasing expression signals over time, as no selection pressure was put on the transfected cells.
pIRES2-ZsGreen1-hBMP2 dual expression plasmid containing both ZsGreen1 GFP reporter gene and BMP2 functional gene was fabricated to monitor the transgene expression level.
FMI can also be used in transgenic detection [ 94, 95], and new probes for molecular imaging can be developed to monitor the transgene expression as well as the activity and function of endogenous genes.
pIRES2-ZsGreen1-hBMP2 dual expression plasmid containing both the ZsGreen1 GFP reporter gene and the BMP2 functional gene was constructed for monitoring the transgene expression level.
As a consequence, the enhancement of tissue-specific expression in the context of Gal4/NF-YA fusion proteins enabled the monitoring of transgene expression using a bioluminescence imaging system.
The application of confocal imaging to in vivo monitoring of transgene expression within internal organs and tissues has been limited by the accessibility to these sites.
