Sentence examples for monitor the transfection from inspiring English sources

To monitor the transfection efficiency, the cancer cells were co-transfected with pEGFP-N1 (Clontech, Mountair View, CA).

To monitor the transfection efficiency, Cy3-labeled siRNA targeting lamin A/C was transfected into cells in parallel in all transfections, and at least 80% transfection efficiency was achieved.

A GFP-2A-Puromycin resistant gene expression cassette was placed after the gRNA cassette both to monitor the transfection efficiency and for selection (Fig. 2A).

To monitor the transfection efficiency, we used FITC-labeled siRNA.

A fluorescein conjugated non-targeting siRNA (Santa Cruz) and a GFP-encoding control plasmid was used to monitor the transfection efficiency.

A β-galactosidase-expressing plasimd was included in each transfection to monitor the transfection efficiency.

For monitoring the transfection efficacy we used non-specific 21 base pairs long DY-547 labelled siRNA duplex (siGlo) provided by Dharmacon (Lafayette, USA).

Red UC luminescence of UCNPs are able to track the delivery of nanocapsules in cells without background fluorescence interference, in the meantime, the green fluorescence of green fluorescence protein (GFP) expressed by the pDNA could subtly monitor the gene transfection efficacy.

Essentially the transfection efficiency of the different RNAi probes was similar as was that of the BLOCK-IT "empty vector control" which was used to monitor the efficiency of transfection.

Relative luciferase activity (Luc) calculated by the ratio of Firefly and Renilla luciferase signals was used to monitor the efficiency of transfection.

In this way, detection of GFP fluorescence allowed us to monitor the efficiency of transfection and the changes that occurred to p62-positive aggregates.