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It was more practical to monitor the transcript levels of 10 50 genes under the various conditions or at different times than the real-time RT-PCR, DNA microarray and SAGE.
DNA microarrays are now routinely used to monitor the transcript levels of thousands of genes simultaneously.
DNA microarrays have emerged as a powerful tool to monitor the transcript levels of thousands of genes simultaneously [ 1, 2].
We used rice whole-genome Agilent oligoarrays (4×44K) to monitor the transcript profiles of the leaves of two pairs of rice NILs responding to WD stress.
Consequently, these primers should monitor the transcript levels of each contig encoding CHS but not the combined expression of both genes (Additional file 8).
We conducted RT-PCR analyses to monitor the transcript levels of seven TaCBL and 17 TaCIPK genes under abiotic stress and hormone treatments.
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We monitored the transcript levels of PdPR5-1 during fruit maturation and ripening as well as after M. fructicola infection.
Moreover, we monitored the transcript abundances of apple MLO homologs following P. leucotricha inoculation in three apple cultivars.
Consistent results were also obtained when we confirmed the luciferase expression data by monitoring the transcript profile of these genes under LL.
To differentiate these two functions, we monitored the transcript levels of genes representative of each pathway in both symbiotic and aposymbiotic insects using RT-qPCR (Fig. 3).
We stimulated HEK 293 cells for 6 hours with either Wnt3A-conditioned medium, or with LiCl (an inhibitor of GSK3β), and we monitored the transcript levels of these genes by RT-qPCR.
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