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In order to monitor the specificity of CKII phosphorylation, 4,5,6,7- tetrabromobenzotriazole (TBB), a specific inhibitor of CKII [34], was utilized.
Thermal conditions were: 94°C for 10 s as the first denaturing step, followed by 94°C for 5 s, 55°C to 60°C for10 s, and 72°C for 15 s, for 40 cycles, followed by a gradual increase in temperature from 55°C to 96°C during the dissociation stage (to monitor the specificity of each primer set).
Mouse melanoma B16F10 cells are HPV-negative and were used to monitor the specificity of Cidofovir action.
In a first series of experiments, we explored the contribute of spatial factors in time processing (past vs. future) using verbs and modifying their tense to monitor the specificity of semantic motor activations for spatial representation of past and future.
At the end, samples were subjected to a slow temperature-ramping dissociation stage of 15 seconds at 95°C, 15 seconds at 60°C, 15 seconds at 95°C to monitor the specificity of amplification.
To monitor the specificity of reaction, IgG was used as an isotype control.
Similar(48)
We first monitored the specificity of the knockdown effects.
Accordingly, a melting curve was generated at the end of the PCR amplification for monitoring the specificity of PCR reaction.
Both Lux™ and Plexor™ technologies allow for dissociation curves to be analyzed, additionally monitoring the specificity of the product.
While in our study we used 10 model substrates to monitor the substrate specificity of FAEs there are more than 30 substrates where FAEs have demonstrated activities [17] and is general belief that there are many more to be discovered.
The reason for using two dyes instead of one was to monitor the incorporation specificity.
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