Promoter GFP fusions were constructed to monitor the regulation of glycolytic, gluconeogenenic and glyoxylate cycle genes in C. albicans (Fig. 1).
The Toll-like receptor agonists Pam3CSK4, flagellin, zymosan and LPS all induced an up-regulation of GMRα surface expression, validating the use of GM-Fc as a sensitive way to monitor the regulation of GMRα expression by immune cells.
In order to monitor the regulation of myogenic cell differentiation and fusion by various extrinsic factors, we generated transgenic trout (Oncorhynchus mykiss) carrying a construct containing the green fluorescent protein reporter gene driven by a fast myosin light chain 2 (MlC2f) promoter, and cultivated genetically modified myogenic cells derived from these fish.
Future studies could investigate this hypothesis by monitoring the regulation of genes associated with calcium signaling such as aggrecan, type II collagen, link protein, and matrix metalloproteinases (Fitzgerald et al. 2004) under the mechanical loads applied here to determine if the metabolic response is primarily anabolic or catabolic.
Activity related to self-monitoring and the regulation of responses, processes which are essential to the act of deception, was also observed.
To be able to monitor the activity and regulation of individual isoforms of the nitrate and peptide transporter family in planta and to study their structure function relationships, we engineered five transporters of the NPF/POT family to report their activity and conformation in vivo.
Therefore, we added them to the selection of network nodes, because marker genes help to monitor the effects of regulation by miRNAs and transcription factors on chondrogenic differentiation.
Interestingly, our findings are in close agreement with a recent study, which monitored the up-regulation of genes involved in UPR, ERAD, and ER-phagy in P. pastoris expressing different lysozyme variants (28); in both cases, a strong correlation was observed between the decrease in lysozyme native stability and an increase in UPR activation.
This system has great advantage, 1) to monitor the dynamism of splicing regulation in vivo with single cell resolution, 2) to identify essential cis-elements and trans-factors, and reveal the hidden mechanisms of splicing regulation like this study, 3) to identify the essential candidate factors by fluorescent color change or to screen the regulators using cDNA or siRNA library.
According to this Regulation, it is mandatory to perform a 90-day rodent feeding study for single transformation events, whereas "the Commission shall monitor the application of this Regulation, the developments in scientific knowledge on replacement, reduction and refinement of animal use in scientific procedures and the publication of new guidance from EFSA.
politicalactivist writes: "As a result of legal political activities, I am fairly certain that I am monitored under according to the Regulation of Investigatory Powers Act.
