Sentence examples for monitor the purification from inspiring English sources

To monitor the purification process we developed a novel HPLC activity assay where a sequential order of phosphorylation was observed whereby the first tyrosine residue was completely phosphorylated prior to phosphorylation of the tandem tyrosine residue.

SELDI was used to monitor the purification.

We used the most abundant specific peptides of the mouse monoclonal anti-DNP to monitor the purification procedure.

Periodically, the activity of NOX and the concentration of proteins were analyzed in both the supernatant and the suspension fractions to monitor the purification process.

To monitor the purification procedure, samples were taken of each fraction and analyzed by sodium dodecyl sulfate-poly acrylamide gel electroforese (SDS-PAGE) and protein staining.

An antiserum from a human melanoma patient, which detected melanoma antigens expressed on a large proportion of different melanoma cells, was used in leucocyte-dependent cytotoxic antibody (LDA) 51Cr-release assays to monitor the purification of melanoma antigens in urea/acetate extracts of lactoperoxidase 125I-labelled melanoma cell membranes.

Gel permeation chromatography and nuclear magnetic resonance were used to monitor the cyclization and purification procedures.

Aliquots of each fraction were analyzed by SDS PAGE (staining with Coomassie Brilliant Blue R-250) to monitor the progress of purification.

This allowed us to monitor the efficiency of RNA purification and cDNA synthesis steps, and to reveal the presence of PCR inhibitors in the samples [64].

After incubation, the solutions were analyzed by gel electrophoresis without purification to monitor the separated strands.

First, we used immunoblotting to monitor the subcellular distribution of Rab26 during the purification of synaptic vesicles from the rat brain.