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Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells.
One of the main applications of the transient gene assay tools is to monitor the localization of proteins to sub-cellular compartments in living cells.
While we did not monitor the localization of ICP0 within our previous study, others have shown that ICP0 RING finger mutants localize predominantly within the nucleus [22], [63].
To answer both of these questions, we utilized the SEAP vector described above to monitor the localization of COPI and COPII vesicle marker proteins with respect to secretory pathway cargo, in this case again monitoring SEAP itself as the cargo, and alterations that occur due to the presence of p22.
To monitor the localization of prelamin A/C, cells were transfected with DsRed-prelamin A/C.
Confocal fluorescence microscopy was used to monitor the localization of MET, RON and PAX8.
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On the contrary, here we aimed to perform our experiments respecting the physiological stoichiometry of protein/mRNA interactions by monitoring the localization of native RNAs and protein.
To determine the subcellular localization of AtGILP, we constructed a fusion of the green fluorescent protein (GFP) gene and AtGILP under the control of the CaMV 35S promoter and monitored the localization of GFP-AtGILP in Arabidopsis mesophyll protoplasts.
We carefully monitored the localization of ICP0 as recent studies have begun to address the biological relevance of its localization during the course of an HSV-1 infection [19], [35], [37], [41], [42].
To test the contribution of all IKK subunits in the activation of NF-κB during infection of epithelial cells by S. flexneri, we monitored the localization of p65 in cells depleted for IKKα, IKKβ or NEMO.
To observe real time dynamics, we monitored the localization of NF-κB in single living cells, which are the basic unit for pathway operations and are the definitive context for probing pathway dynamics unambiguously.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com