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To monitor the induction of immune responses, specific antibody titers in serum as well as secreted mucosal antibodies of local and distal mucosal surfaces were determined.
To monitor the induction of long-lasting tumor protection, mice were re-challenged with 25*103 B16OVA cells 40 days after cryo ablation.
They have also yielded highly-sensitive msn-GFP and msn-DsRed transgenes that can be used to monitor the induction and function of lamellocytes in immune-challenged or genetically-altered Drosophila.
Future studies should seek to use vaccine vectors that increase the magnitude and quality of the IFN-γ immune response in naturally exposed populations and should monitor the induction of a regulatory T cell response.
To monitor the induction of TLR4 signaling in response to ligand stimulation, we have used the pNifty reporter plasmid (Invivogen, Ruschlikon, Switzerland), expressing the secreted embryonic alkaline phosphatase gene (SEAP) under the control of a NF-kB-inducible ELAM1 composite promoter.
To monitor the induction of bacterial lysis, we read the OD600 hourly over a period of 8 hours.
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Differently, the measurement of biosensor responses to specific analyte in soil may allow an online monitoring of the elemental bioavailability by monitoring the induction of specific promoters, allowing a better assessment of bioaccumulation and biomagnification in food chains, and therefore improving the risk assessment for terrestrial ecosystems.
We initially monitored the induction of GFP activity in lymph glands dissected from larvae incubated without or with the parasite.
Moreover, we monitored the induction of apoptosis in confluent HUVEC cultures by radiation (15 Gy) using AnnexinV and 7AAD double staining.
Non-invasive assessment of the dynamics of gene regulation is of interest for the detection of endogenous disease-specific biological alterations (e.g., signal transduction) and for monitoring the induction and regulation of therapeutic genes (e.g., gene therapy).
In contrast to our results, a recent study argues that Wnt signals, but not BMP signals, induce neural crest cells in explants isolated from the intermediate region of the stage 10 caudal neural plate (C) by monitoring the induction of HNK-1 after 48 hr of culture, corresponding in time to stage ∼20 [11].
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