Sentence examples for monitor the folding from inspiring English sources
In this study, directed hydroxyl radical probing mediated by Fe(II -derivatized S20 proteII -derivatized monitor the folding of 16S20RNA during r-proteinsassociation and 30S subunit assembly.
Here we use single-molecule atomic force microscopy to directly monitor the folding and cofactor binding dynamics of an engineered metal-binding protein G6-53 athehe single-molecule level.
The two-dimensional 1H-15N Hspectractra, performed to monitor the folding of the protein mutants, were collected on 100 µM samples of 15N-labeled E,E- and E,Zn-hSOD1SS mutant proteins in 20 mM sodium phosphate buffer (pH 7).
In the UPRmt, molecular chaperones and proteases are modulated to monitor the folding and assembly of mitochondrial proteins.
We have employed far-UV CD, intrinsic tryptophan fluorescence and ANS-binding fluorescence spectroscopy to systematically monitor the folding of C72S as a function of pH.
To monitor the folding and expression of PhoA* in the presence of overexpressed DsbC or DsbG, we transformed cells lacking dsbC and harboring pBAD33-phoA* with the following: (i) an empty expression control vector, (ii) the vector expressing dsbC, or (iii) the vector expressing dsbG-flag.
The heat shock proteins play a key role in cellular metabolism under all growth conditions, monitoring the folding, assembly and translocation of cellular proteins [ 27].
We monitored the fold change in cytokine production compared with baseline (before start of treatment).
GFP was chosen for this assay because it can be detected by fluorescence without antibodies, and also because its auto-fluorescence capability relies on its correct folding, thus allowing us to easily monitor the proper folding of the inclusion-associated tagged GFP.
The last advances in several approaches, which use NMR: (i) to monitor the protein folding pathway and/or, (ii) to characterize the structure of the intermediate states in such reaction are reviewed in this work.
Researchers have identified the cellular components that monitor the protein folding conditions inside the ER.
