Whole-mount in situ hybridization to monitor the expression patterns of the 18-gene NR family (listed in Table S1) in embryos was performed using TSA-FISH, as described previously (Lecuyer et al. 2007, 2008).
To analyze the localization and the expression pattern of AtBTPC in the pollen during pollen development, "fluorescent tagging of full-length proteins technology" was applied, because this strategy was available to monitor the expression patterns and subcellular localization of Arabidopsis gene products in planta [ 26].
To investigate this, we monitored the expression pattern of proIAA2∶GUS in the hyd/fk mutant aerial parts.
Thus, this mouse model allows us to readily assess canonical Wnt β-catenin signalling in vivo by monitoring the expression pattern of LacZ.
To verify the reliability of sequencing-based approach in identifying R. solanacearum -responsive genes, we monitored the expression pattern of twelve candidate DEGs at the five time points post inoculation for both genotypes using qRT-PCR.
In order to determine the effect of glibenclamide on the development of endocrine progenitors, we monitored the expression pattern of Ngn3, which encodes a transcription factor that specifically labels the endocrine progenitors [ 2, 3], before and after 1, 3, 5, and 7 days of culture in the presence or absence of glibenclamide.
To test whether ECs identified in this work could play a role during interaction with host plants, we monitored the expression pattern after plant inoculation by quantitative RT-PCR for 16 EC genes, representative of the four lists of ECs identified through our bioinformatics analyses.
Thus, monitoring the expression patterns of Torvum candidate R genes analogs (RGAs) can help pointing to RGAs of interest [ 51].
The first report monitored the expression patterns of superficial and invasive tumour cell suspensions prepared from 36 normal and 29 bladder tumour biopsies using oligonucleotide microarrays.
