Expression profiling techniques have been used to simultaneously monitor the expression of thousands genes from biological samples.
To monitor the expression of cytokine genes in Japanese pufferfish, a novel platform for quantitative multiplexed analysis was developed.
We attached GFP obtained from the MS2-GFP expression vector39 to the C-terminal region in order to monitor the expression and localisation occurring within a single cell.
Additionally, we successfully utilized the bidirectional expression module to monitor the expression of a suicide gene, showing potential for photodynamic gene therapy.
Experiments were designed to trigger the pathogen-challenged disease responses in both susceptible (H24) and resistant (Anagha) tomato genotypes and monitor the expression of stress induced genes or gene fragments at the transcript level.
Thus, the esterase is suited as a reporter enzyme to monitor the expression of polypeptides coupled to its N-terminus and simultaneously, as a cleavable tag for polypeptide purification.
We used the GUS reporter system to monitor the expression of eight of those genes.
In the current study the reporter strain was used to monitor the expression of the srfA operon which is a known requirement for competence development.
With the advent of DNA hybridisation nanoarrays comes the remarkable ability to rapidly and effectively monitor the expression levels of thousands of genes to diagnose and treat illness [1].
We designed these arrays to monitor the expression of 18,000 genes and associated alternate splicing events [29].
A Ty1-tag (EVHTNQDPLD) [30] at the N-terminus allowed us to monitor the expression of mutant RPB1 proteins.
