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In-situ spectroscopic ellipsometry (SE) in the energy range from 1.5 to 5.5 eV was used to monitor the deposition of chromium nitride coatings deposited by ion-assisted reactive magnetron sputtering.
A series of designed experiments were undertaken to monitor the deposition rate and coating chemistry of both fluorocarbon (heptadecafluorodecyl acrylate) and siloxane (Z-TOMCATS) films.
This configuration enables to deposit the catalyst on chemically clean surface, and at the same time to control the substrate temperature and monitor the deposition process with reflection high-energy electron diffraction.
The radiation from the surface of the growing films detected by a pyrometer and the output power of the substrate temperature controller were used to monitor the deposition process.
The samples were processed to monitor the deposition of proteoglycans and of type-II collagen (toluidine blue staining and immunodetection, respectively) and the expression of SOX9 (immunodetection) (all at magnification ×20; representative data) with corresponding histomorphometric assessments and to estimate the proteoglycan and type-II collagen contents as described in the Methods.
Through the digital thickness monitor, the deposition system allowed the control on the growth rate and overall thickness of thin film during the synthesis.
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Senior S.E.C. lawyers monitor the depositions via videoconference.
To test this, we used a robust assay that monitors the deposition of the transmembrane protein reporter Delta to the plasma membrane [6].
Parallel chromogenic in situ hybridization provided clear labeling for 5-HT1A mRNandnd additionally offered the possibility to monitor the chromogen deposition at regular time intervals to determine the optimal signal-to-noise ratio.
The values of these thicknesses were used as input parameters in our deposition controller, which was based on a quartz microbalance, to monitor the thickness and deposition rate of the Co films.
In this study, XPS analyses were done to monitor the iron oxide deposition in the cross-linked CCIONPs.
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