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The solution was mixed well and heated at 50°C under air in a quartz cell on a UV spectrometer in order to monitor the decrease in the absorbance ofβ-carotene at 460 nm.
Attempts have been made to monitor the decrease in cholesterol biosynthesis by inhibiting the key enzyme, the 3-hydroxy-3-methyl glutaryl coenzyme A (HMG-CoA) reductase.
While the list of chemicals identified as POPs was mainly developed based on ecological monitoring data instead of HBM data, HBM monitoring programs have proven extremely useful to evaluate the efficiency of the Stockholm convention and to monitor the decrease of POPs in the general population [ 65, 66].
To monitor the decrease in sensitivity we analyzed the underivatized polar lipid extract [containing phosphatidylinositol (PI) as well as other polar lipids 3] by negative ion mode FTMS before and after each batch of ten repeated injections of the sulfated sample.
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We quantified the efficiency of targeting by monitoring the decrease in fluorescence within a single cell.
The rate of the thermal fading reaction was examined by monitoring the decrease in the absorbance at 556 nm after cessation of illumination (Fig. 1d).
Additionally, we investigated the possibility of collisional quenching of triethylamine by monitoring the decrease in fluorescence emission of photoactivated 1, rhodamine B and rosamine (Supplementary Fig. 13).
Thus, as the pH of the pre-gelation solution slowly drops over time, the self-assembly of each gelator can be tracked by monitoring the decrease in the integral values of characteristic 1H NMR peaks50.
The replacement of Mant-ADP by ATP was detected by monitoring the decrease in fluorescence at 440 nm by adding Mg-ATP at various concentrations to the mixture of ΔN-TClpB (1 μM as hexamer) and 12.5 μM Mant-ADP.
The sensing mode is based on monitoring the decrease of the amperometric signal corresponding to the electrochemical reduction of dopaquinone enzymatically generated.
By using such method, AGR2 could be sensitively detected in the range of 5.0 pM–1.0 nM based on monitoring the decrease of the fluorescence signal.
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