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The fluorescence emission of the tryptophan residue was used to monitor the binding of peptides to liposomes, since fluorescence of the tryptophan residue is sensitive to different environments.
Surface plasmon resonance (SPR) biosensors are label free detection systems that monitor the binding of specific biomolecular recognition elements with binding partners.
We have developed a surface plasmon resonance (SPR) biosensor-based assay to monitor the binding of the sera anti-VR Abs to the parental Ab and the inhibition of this binding by the variants.
To monitor the binding process by flow cytometry, both the reporter probes and internal control probes were conjugated with Quantum dots that fluoresce at different emission wavelengths using the click reaction.
Here, alternative methods are used to characterize the structural properties of the RAGE-ligand binding domain and to monitor the binding of a series of truncated variants of Aβ.
In order to determine the precise kinetic parameters, we selected a multichannel QCM sensing system to monitor the binding reaction of PhaR from Ralstonia eutropha H16 to target DNAs (including the promoter regions of phaP and phaR) and thin films of amorphous P 3HB) [am-P 3HB)] derived from atactic P(3HB).
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Third, the assay employed by Metushi et al. [42] monitored the binding affinity of the peptide towards the HLA, not the actual binding affinity of the drug.
The charge conduction properties of DNA can be harnessed for monitoring the binding of a ligand to its receptor.
It can also be used to investigate the effectiveness of early-phase treatment response by monitoring the binding of drug molecules to the tumor cells.
The grafting of biotin OEG NH2 was assessed by monitoring the binding of neutravidin and albumin to the biotinylated surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D), as well as by PM-RAIRS.
Fluorescence titration experiments monitoring the binding of oligopyrimidines to Bs-CspB reveal binding preferences at individual subsites and allow the design of an optimised heptapyrimidine ligand, which is bound with sub-nanomolar affinity.
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