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In this way, it was possible to monitor the bacterial growth by the online acquisition of NaOH addition.
In an independent experiment, 2 mice infected as described (untreated mice) and 2 mice infected and then treated as above (treated mice) were sacrificed at daily intervals to monitor the bacterial load in the target organs (liver and GI tract of mice infected with S. enterica serovar Paratyphi; the kidneys of mice infected with S. aureus).
To monitor the bacterial invasion of the fungus, we performed several microscopic investigations using GFP-labeled bacteria.
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The bacterial growth curves were then plotted against time based on the data obtained by carefully monitoring the bacterial growth.
The 2C4NP degradation ability of strain CNP-8 was determined by monitoring the bacterial growth (OD600) and 2C4NP consumption.
Present finding gives an insight towards scaling up siderophore production from P. aeruginosa strain EGYII DSM 101801, where, testing different agitation speeds in the fermentor alongside monitoring the bacterial growth and the siderophore production would be a mandatory task to help mediate a short log phase and achieving to an earlier stationary phase.
Thus, in future studies, monitoring the bacterial composition of GCF may give information of the current activity of the subgingival biofilm in vivo.
To test this hypothesis, we added different amounts of spermidine (up to 1 mM) to the LB medium, and monitored the bacterial growth by measuring absorbance at 600 nm and the expression of exsCEBA in strains PAO1 and ΔspuE by measuring the β-galactosidase activity encoded by the pC-lacZ reporter gene.
The effect of immunization was first evaluated by monitoring the bacterial burden in vaginal swabs collected every 3 days as reported [20].Naïve, non-immune C57BL/6 mice develop an immune response against C. muridarum and typically clear a genital infection 2 3-wks later [35] as was observed in mice given GL-vaults (Fig. 7).
PTSs play a major role in uptake and phosphorylation of numerous carbohydrates, as well as in monitoring the bacterial environment to choose alternative carbon sources for growth [ 33].
Stage 11 embryos were also not able to contain an infection, as revealed by monitoring the bacterial load as early as 8 hpi and their survival to the first-instar larval stage postinfection with bacterial species considered to be nonpathogenic in larvae and adults.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com