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Moreover, the designed biosensor was able to monitor release of IP3 upon induction by different inducers like Bradykinin and ATP.
These models must also incorporate changes in release due to the dissolution media and methods employed to monitor release.
Here we show that focused ultrasound, used at pressures similar to those applied during diagnostic ultrasound scanning, can be utilised to both trigger and monitor release of payload from liposomes.
These were observed daily to monitor release of ascospores, usually signalled by white spore shadows on the agar surface and/or on the lid.
After 24 h, the conditioned medium was removed, centrifuged in a Beckman microfuge for 5 min and counted in a β-scintillation counter to monitor release of soluble [H]-collagen fragments from the insoluble matrix.
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